Alignment of recombination sites in Hin-mediated site-specific DNA recombination.

Utilization of Site-Specific Recombination in Biopharmaceutical Production

Mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. Several recombinant protein systems have been introduced to the market, most of which are under clinical development. In spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

The role of negative supercoiling in Hin-mediated site-specific recombination.

A series of biochemical assays were developed and performed to monitor the molecular events that occur during the Hin-mediated DNA inversion reaction. These events can be divided into five different stages: 1) binding of proteins (Hin, Fis, and HU) to DNA; 2) pairing of Hin-binding sites; 3) invertasome formation; 4) DNA strand cleavage; 5) strand rotation and religation. A series of topoisomer...

utilization of site-specific recombination in biopharmaceutical production

mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. several recombinant protein systems have been introduced to the market, most of which are under clinical development. in spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

Site‐specific recombination

Symmetric DNA sites are functionally asymmetric within Flp and Cre site-specific DNA recombination synapses.

Flp and Cre-mediated recombination on symmetrized FRT and loxP sites, respectively, in circular plasmid substrates yield both DNA inversion and deletion. However, upon sequestering three negative supercoils outside the recombination complex using the resII-resIII synapse formed by Tn3 resolvase and the LER synapse formed by phage Mu transposase in the case of Flp and Cre, respectively, the reac...